Zebra Grant Project Comparison of Albumin Measurements by Electrophoresis and the Bromocresol Green Method in Chicken Plasma
نویسنده
چکیده
Avian species are renowned for their ability to conceal clinical evidence of disease until the pathological process is advanced. As in mammalian species, total protein (TP) measurement forms part of any routine biochemistry profile, often evaluated in conjunction with other biochemical and haematological parameters to assess the health status of the bird. However, significant variation in protein fractions may occur in the absence of alterations in TP, creating a need for a reliable method of evaluating the individual components.
منابع مشابه
The introduction of bromocresol purple for the determination of serum albumin on SMAC and ACA, and the standardization procedure.
The bromocresol purple methods for the determination of serum albumin on the Technicon SMAC and the DuPont ACA systems were evaluated and compared with the corresponding bromocresol green methods, a radial immunodiffusion (RID) and an electrophoresis technique. The linearity and precision of the dye binding methods were comparable. The bromocresol purple methods were more influenced by lipaemia...
متن کاملImmunoglobulin Classes and Nutritional Factors in Plasma and Breast Milk of Lactating Mothers in Nigeria
Background: Breast milk is important for the overall well-being of infants. Although lactation is relatively robust in the face of poor nutrition, the implication of poor nutrition on non-nutritive factors in breast milk is inconclusive. Objective: This study was designed to find associations between nutritional and immune factors in maternal blood and breast milk with the aim to improve the ne...
متن کاملMeasurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods.
BACKGROUND/AIMS The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), ...
متن کاملIsolation and purification of albumin from human plasma by direct and combined approach of ion-exchange chromatography and comparison of the final products quality obtained by both methods
Background: Due to multiple roles of albumin in the body, injection of its medicinal product as one of the therapeutic or management strategies under conditions such as severe bleeding, burns, liver failure, and neonatal hemolytic diseases is on the physicians' agenda. Considering that albumin is the most abundant plasma protein, designing an appropriate method to purify it is highly important....
متن کاملBromocresol purple, a non-specific colour reagent for the determination of serum albumin.
The determination of serum albumin with the dye reagent bromocresol purple was investigated. We found that bromocresol purple is not a specific reagent for albumin, but that serum proteins in the alpha-, beta-, and gamma-globulin fractions also react with this dye. Similar results were obtained for bromocresol green. Furthermore there is a great difference in absorbance between human and bovine...
متن کامل